Journal: Cancers

Article Title: Nanoparticles as Physically- and Biochemically-Tuned Drug Formulations for Cancers Therapy

doi: 10.3390/cancers14102473

Figure Lengend Snippet: Examples of nanoparticles responsive to biochemical features of the tumor microenvironment.

Article Snippet: Testing the derived nanoparticles revealed the optimal performance of diselenide bond-including paclitaxel dimers (in terms of response to reductive conditions) included in the Pluronic ® F127 nanocarrier (in terms of accumulation at the tumor site and therapeutic effect in a xenograft model of triple-negative breast cancer).

Techniques: Starch

Journal: Cancers

Article Title: Nanoparticles as Physically- and Biochemically-Tuned Drug Formulations for Cancers Therapy

doi: 10.3390/cancers14102473

Figure Lengend Snippet: Examples of antibody fragment-functionalized nanoparticles for targeted drug delivery.

Article Snippet: Testing the derived nanoparticles revealed the optimal performance of diselenide bond-including paclitaxel dimers (in terms of response to reductive conditions) included in the Pluronic ® F127 nanocarrier (in terms of accumulation at the tumor site and therapeutic effect in a xenograft model of triple-negative breast cancer).

Techniques: In Vitro, In Vivo

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Nitric Oxide-Releasing Nanoscale Metal-Organic Layer Overcomes Hypoxia and Reactive Oxygen Species Diffusion Barriers to Enhance Cancer Radiotherapy.

doi: 10.1002/advs.202413518

Figure Lengend Snippet: Figure 4. In vitro anticancer effect of SNAP/MOL under X-ray irradiation. a) Cellular uptake of MOL and SNAP/MOL determined by ICP-MS. b) Clono- genic assays to evaluate radioenhancement of MOL and SNAP/MOL in 4T1 cells. n = 3. c) Quantitation of cell apoptosis and necrosis after different treatments. n = 3. d) Representative flow cytometry results showing cell apoptosis and necrosis co-stained with Annexin-V and PI. e) CLSM images, f) western blot results, and g) flow cytometry analysis of 𝛾-H2AX signals after different treatments. All data are shown as mean±SD.

Article Snippet: Murine colorectal carcinoma CT26 and murine triple negative breast cancer 4T1 cell lines were purchased from the American Type Culture Collection (ATCC, Rockville, MD), and cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS) and penicillin G sodium (100 U mL−1) and streptomycin sulfate (100 μg mL−1) in a humidified atmosphere with 5% CO2 at 37 °C.

Techniques: In Vitro, Irradiation, Quantitation Assay, Cytometry, Staining, Western Blot

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Nitric Oxide-Releasing Nanoscale Metal-Organic Layer Overcomes Hypoxia and Reactive Oxygen Species Diffusion Barriers to Enhance Cancer Radiotherapy.

doi: 10.1002/advs.202413518

Figure Lengend Snippet: Figure 5. Mechanistic studies of radiosensitization of SNAP/MOL. CLSM images showing distribution of a) ONOO−and b) ROS in 4T1 cells after SNAP/MOL(+) treatment. c) Colocalization between nucleus (blue) and ONOO−(red) in the region of interest (ROI, white arrow in Figure 5a). d) Colocalization between nucleus (blue) and ROS (green) in the ROI (white arrow in Figure 5b). e) Relative nucleus distribution (RND) statistics for ONOO−and ROS. n = 10. f) Flow cytometry results showing the hypoxia level of 4T1 cells after different treatments. g) Intracellular ATP levels of 4T1 cells after different treatments. f,g, data are presented as mean ± SD (n = 3). Statistical significance was calculated via unpaired two-tailed student’s t test. *p<0.05, ***p<0.001.

Article Snippet: Murine colorectal carcinoma CT26 and murine triple negative breast cancer 4T1 cell lines were purchased from the American Type Culture Collection (ATCC, Rockville, MD), and cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS) and penicillin G sodium (100 U mL−1) and streptomycin sulfate (100 μg mL−1) in a humidified atmosphere with 5% CO2 at 37 °C.

Techniques: Flow Cytometry, Two Tailed Test

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Nitric Oxide-Releasing Nanoscale Metal-Organic Layer Overcomes Hypoxia and Reactive Oxygen Species Diffusion Barriers to Enhance Cancer Radiotherapy.

doi: 10.1002/advs.202413518

Figure Lengend Snippet: Figure 6. In vivo antitumor effects. a) Tumor growth profiles of CT26-tumor-bearing mice after different treatments. b) Resected CT26 tumor weights on day 21. c) Tumor growth profiles of 4T1-tumor-bearing mice with various treatment regimens. d) Resected tumor weights of 4T1 tumor-bearing BALB/c mice at the end of therapies. e) TUNEL, g) H&E and h) 𝛾-H2AX staining of excised 4T1 tumors. Scale bars, 50 μm. Quantification of positive cell percentage in IHC staining of f) TUNEL and i) 𝛾-H2AX for evaluation of DNA damage and apoptosis. All data are shown as mean±SD, n = 5. Statistical significance was calculated via unpaired two-tailed student’s t test. *p<0.05, **p< 0.01, ***p<0.001.

Article Snippet: Murine colorectal carcinoma CT26 and murine triple negative breast cancer 4T1 cell lines were purchased from the American Type Culture Collection (ATCC, Rockville, MD), and cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS) and penicillin G sodium (100 U mL−1) and streptomycin sulfate (100 μg mL−1) in a humidified atmosphere with 5% CO2 at 37 °C.

Techniques: In Vivo, TUNEL Assay, Staining, Immunohistochemistry, Two Tailed Test

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Nitric Oxide-Releasing Nanoscale Metal-Organic Layer Overcomes Hypoxia and Reactive Oxygen Species Diffusion Barriers to Enhance Cancer Radiotherapy.

doi: 10.1002/advs.202413518

Figure Lengend Snippet: Figure 7. Pulmonary metastasis assessment and mechanistic summary. a) Representative images of H&E staining of lungs of 4T1-tumor-bearing BALB/c mice after different treatments. b) Statistical analysis of pulmonary metastatic nodules in different groups. n = 4. c) NO level in tumor tissues mea- sured by CLSM using probe DAF-FM. d) Representative IHC staining of HIF-1𝛼(brown) in excised 4T1 tumors after different treatments. Scale bars, 50 μm. e) Quantification of HIF-1𝛼positive cells by IHC staining to assess tumor hypoxia. n = 5. f) Schematic presentation of SNAP/MOL-mediated radiosensitization. Under low-dose X-ray irradiation, SNAP/MOL releases NO and ROS in a spatiotemporally synchronized manner, leading to efficient generation of long-lived and highly toxic ONOO−, thereby increasing DNA damage. Meanwhile, the sustained release of NO in the TME relieves tumor hypoxia and reduces radioresistance of tumor cells. Figure 7f was created with BioRender.com. All data are shown as mean±SD. Statistical significance was calculated via unpaired two-tailed student’s t test. *p<0.05.

Article Snippet: Murine colorectal carcinoma CT26 and murine triple negative breast cancer 4T1 cell lines were purchased from the American Type Culture Collection (ATCC, Rockville, MD), and cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS) and penicillin G sodium (100 U mL−1) and streptomycin sulfate (100 μg mL−1) in a humidified atmosphere with 5% CO2 at 37 °C.

Techniques: Staining, Immunohistochemistry, Irradiation, Two Tailed Test

Journal: Bioorganic chemistry

Article Title: Novel steroidal oximes as antiproliferative agents: Design, synthesis and biological activity evaluation.

doi: 10.1016/j.bioorg.2025.108229

Figure Lengend Snippet: Fig. 2. Influence of OX1 and EP2OX on the H1299, PC3 and HCC1806 cells distribution by the different phases of the cell cycle. The cells were treated for 48 h with two different concentrations: one corresponding to the IC50 of each compound in each cell line and the other superior to the IC50 – 50 µM for OX1 and 15 µM for EP2OX. Results of the cell cycle express the percentage of cells in Sub-G1, G0/G1 phase, S phase and G2/M phase and represent the mean ± SD of four independent experiments. Significant differences are denoted by *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.

Article Snippet: The tumour cell lines HCC1806 (triple-negative breast cancer), H1299 (lung cancer) and PC3 (prostate cancer) and the non-tumorigenic cell line MRC-5 (lung tissue fibroblasts) were acquired from American Type Collection Culture (ATCC, Rockville, MD, USA).

Techniques:

Journal: Bioorganic chemistry

Article Title: Novel steroidal oximes as antiproliferative agents: Design, synthesis and biological activity evaluation.

doi: 10.1016/j.bioorg.2025.108229

Figure Lengend Snippet: Fig. 3. Viability and cell death profile induced by OX1 and EP2OX in H1299, PC3 and HCC1806 cancer cells. The cells were treated for 48 h with two different concentrations: one corresponding to the IC50 of each compound in each cell line and the other superior to the IC50 – 50 µM for OX1 and 15 µM for EP2OX. Results are presented as a percentage (%) of viable cells (V), in early apoptosis (A), in late apoptosis/necrosis (A/N) and necrosis (N) and express the mean ± SD of, at least, four independent experiments. Significant differences are denoted by *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.

Article Snippet: The tumour cell lines HCC1806 (triple-negative breast cancer), H1299 (lung cancer) and PC3 (prostate cancer) and the non-tumorigenic cell line MRC-5 (lung tissue fibroblasts) were acquired from American Type Collection Culture (ATCC, Rockville, MD, USA).

Techniques:

Journal: Bioorganic chemistry

Article Title: Novel steroidal oximes as antiproliferative agents: Design, synthesis and biological activity evaluation.

doi: 10.1016/j.bioorg.2025.108229

Figure Lengend Snippet: Fig. 6. DNA damage assessment induced by EP2OX in H1299, PC3 and HCC1806 cancer cells. A – Levels of γH2AX, 48 h after treatment with EP2OX, at two different concentrations: one corresponding to the IC50 of each cell line and the other higher than the IC50 (15 µM). Expression levels are presented normalized to control (dashed line). Results express the mean ± SD of, at least, four independent experiments. B – DNA damage in H1299 cancer cells using halo assay, 48 h after treatment with EP2OX at two different concentrations: 1.13 and 2.50 µM. Representative images of non-treated cells; positive control: cells treated with H2O2 at 400 µM; cells treated with EP2OX at 1.13 µM; cells treated with EP2OX at 2.50 µM. Halo images were analyzed using HaloJ plugin in ImageJ. Bar: 20 µm. Magnification: 20Х. C – Percentage of DNA in halo nuclei and NDF. Results present the median of three independent experiments, obtained by quantifying 75–120 images using the HaloJ program. Significant differences are denoted by *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 for both experiments.

Article Snippet: The tumour cell lines HCC1806 (triple-negative breast cancer), H1299 (lung cancer) and PC3 (prostate cancer) and the non-tumorigenic cell line MRC-5 (lung tissue fibroblasts) were acquired from American Type Collection Culture (ATCC, Rockville, MD, USA).

Techniques: Expressing, Control, Halo Assay, Positive Control

Journal: Macromolecular Bioscience

Article Title: Evaluation of HER2 Targeted Poly(Ethylene Glycol)‐Poly( L ‐histidine) Copolymer Micelles in Breast Cancer Models

doi: 10.1002/mabi.202500047

Figure Lengend Snippet: Cellular association of PEG‐PHis‐RB micelle variants after 3 or 24 h incubation. Cells were incubated at 37°C with PEG‐PHis‐RB micelles with or without TZM conjugation either for 3 or 24 h, respectively. Cells were harvested and analyzed by flow cytometry. 5000—10 000 events were collected per sample. Mean fluorescence intensity (MFI) with respect to RB for (A) SKBR3 cells and (B) JIMT1 cells is presented here as % of untreated control ± SEM of 2 or 3 independent experiments, each performed with either duplicates or triplicates. Data are analyzed by multiple t‐ tests for 3 h, and two‐way ANOVA for 24 h. * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: JIMT‐1 cells (ER‐, PR‐, HER2+) were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany) and cultured using a 1:1 mixture of DMEM/F‐12 medium with the same supplements as mentioned above.

Techniques: Incubation, Conjugation Assay, Flow Cytometry, Fluorescence, Control

Journal: Macromolecular Bioscience

Article Title: Evaluation of HER2 Targeted Poly(Ethylene Glycol)‐Poly( L ‐histidine) Copolymer Micelles in Breast Cancer Models

doi: 10.1002/mabi.202500047

Figure Lengend Snippet: Effects of empty PEG‐His micelles with or without TZM conjugation on cell lines with varying HER2 expression levels. SKBR3 (HER2 high) or JIMT1 cells (HER2 moderate) were treated with empty PEG‐PHis micelle variants at a concentration equivalent to 4 µg/mL TZM. Untreated cells and treatment with free TZM were set as controls. (A) % cell confluence normalized to day 0 of treatment (B) Representation of the values 3 days after treatment, showing statistical significance. (C) MTT assay performed after 3 days with the same treatments. Values represent mean ± SD. Here, statistical analysis between control vs. TZM in SKBR3 cells has been performed by an unpaired t ‐test with Welch correction. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: JIMT‐1 cells (ER‐, PR‐, HER2+) were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany) and cultured using a 1:1 mixture of DMEM/F‐12 medium with the same supplements as mentioned above.

Techniques: Conjugation Assay, Expressing, Concentration Assay, MTT Assay, Control

Journal: Frontiers in Immunology

Article Title: Hyaluronic Acid Present in the Tumor Microenvironment Can Negate the Pro-apototic Effect of a Recombinant Fragment of Human Surfactant Protein D on Breast Cancer Cells

doi: 10.3389/fimmu.2020.01171

Figure Lengend Snippet: Flow cytometric analysis of apoptosis induction in breast cancer cells treated with 20 μg/ml of immobilized and solution-phase rfhSP-D for 24 h.

Article Snippet: Human breast cancer cell lines, triple-negative BT20 (ER − /PR − /HER2 − ) (ATCC-HTB19), triple-positive BT474 (ER + /PR + /HER2 + ) (ATCC-HTB20), and HER2-positive SKBR3 (ER − /PR − /HER2 + ) (ATCC-HTB30), were cultured in complete RPMI medium (Sigma-Aldrich), supplemented with 10% v/v fetal bovine serum (FBS), 2 mM l -glutamine, 100 U/ml penicillin (Sigma-Aldrich), 100 μg/ml streptomycin (Sigma-Aldrich), and 1 mM sodium pyruvate (Sigma-Aldrich) and left to grow at 37°C under 5% v/v CO 2 .

Techniques:

Journal: Frontiers in Immunology

Article Title: Hyaluronic Acid Present in the Tumor Microenvironment Can Negate the Pro-apototic Effect of a Recombinant Fragment of Human Surfactant Protein D on Breast Cancer Cells

doi: 10.3389/fimmu.2020.01171

Figure Lengend Snippet: Apoptosis induction in BT20, BT474, and SKBR3 cell lines following HA challenge in the presence and absence of rfhSP-D (A) . The data were expressed as the mean of three independent experiments ( n = 3). A significant difference was seen among treated and untreated samples, as made evident by the shift in the fluorescence intensity. Staurosporine (1 μM/ml) was used as a positive control. Proliferative effects of rfhSP-D treatment on BT474 and SKBR3 breast cancer cell lines (B) . BT474 and SKBR3 cells were seeded in wells pre-coated with HA, HA + rfhSP-D, and rfhSP-D alone. The percentage of proliferative cells was evaluated by staining with mouse anti-human KI-67 antibody, and KI-67-stained cells were measured via flow cytometry. The data were generated from at least three independent experiments ( n = 3) and presented as mean ± SD (* p < 0.1, ** p < 0.01, and **** p < 0.0001). The statistical analysis was performed between rfhSP-D and HA + rfhSP-D-treated breast cancer cells. The secretion levels of FL-SP-D were confirmed and analyzed via western blotting (C) . Culture medium collected from BT20, BT474, and SKBR3 cell lines was passed through a maltose Agarose column, and the eluted fractions were validated via western blotting; FL-SP-D was detected at ~43 kDa only for BT474. Both BT20 and SKBR3 cell lines did not secrete any FL-SP-D. Secreted FL-SP-D by BT474 was tested for its ability to induce apoptosis (D) . No effect of secreted FL-SP-D was seen in terms of cell viability and apoptosis induction.

Article Snippet: Human breast cancer cell lines, triple-negative BT20 (ER − /PR − /HER2 − ) (ATCC-HTB19), triple-positive BT474 (ER + /PR + /HER2 + ) (ATCC-HTB20), and HER2-positive SKBR3 (ER − /PR − /HER2 + ) (ATCC-HTB30), were cultured in complete RPMI medium (Sigma-Aldrich), supplemented with 10% v/v fetal bovine serum (FBS), 2 mM l -glutamine, 100 U/ml penicillin (Sigma-Aldrich), 100 μg/ml streptomycin (Sigma-Aldrich), and 1 mM sodium pyruvate (Sigma-Aldrich) and left to grow at 37°C under 5% v/v CO 2 .

Techniques: Fluorescence, Positive Control, Staining, Flow Cytometry, Generated, Western Blot

Journal: Frontiers in Immunology

Article Title: Hyaluronic Acid Present in the Tumor Microenvironment Can Negate the Pro-apototic Effect of a Recombinant Fragment of Human Surfactant Protein D on Breast Cancer Cells

doi: 10.3389/fimmu.2020.01171

Figure Lengend Snippet: Intracellular signaling to show phosphorylation of p53 in rfhSP-D-treated BT20 and SKBR3 cell lines (A) . Breast cancer cell lines were allowed to adhere to HA or HA-bound rfhSP-D, and the phosphorylation status of p53 was evaluated using total cell lysates with a PathScan Antibody Array Kit (Cell Signaling). Data were generated from at least three independent experiments and presented as mean ± SD. rfhSP-D treatment resulted in upregulation of p21 and p27 cell cycle inhibitors (B) . BT20, BT474, and SKBR3 (0.4 × 10 6 ) cells were seeded in a six-well-plate pre-coated with rfhSP-D and HA + rfhSP-D. The treated cells were lysed and pelleted down. The pelleted cells were subjected to RNA isolation, followed by cDNA synthesis and qPCR. The comparative quantification method was performed to calculate the efficiencies of each gene for each individual PCR and is based on the second differential maximum method or takeoff analysis. The takeoff results obtained with p21/p27 primers were normalized with the housekeeping gene TBP. qPCR assay was conducted in triplicates, and error bars represent ± SEM. Unpaired one-way ANOVA test was used to determine the significance; * p < 0.05, ** p < 0.01, and *** p < 0.001 ( n = 3). The statistical analysis was performed between rfhSP-D and HA + rfhSP-D-treated breast cancer cells.

Article Snippet: Human breast cancer cell lines, triple-negative BT20 (ER − /PR − /HER2 − ) (ATCC-HTB19), triple-positive BT474 (ER + /PR + /HER2 + ) (ATCC-HTB20), and HER2-positive SKBR3 (ER − /PR − /HER2 + ) (ATCC-HTB30), were cultured in complete RPMI medium (Sigma-Aldrich), supplemented with 10% v/v fetal bovine serum (FBS), 2 mM l -glutamine, 100 U/ml penicillin (Sigma-Aldrich), 100 μg/ml streptomycin (Sigma-Aldrich), and 1 mM sodium pyruvate (Sigma-Aldrich) and left to grow at 37°C under 5% v/v CO 2 .

Techniques: Phospho-proteomics, Ab Array, Generated, Isolation, cDNA Synthesis